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Magnesium Chloride Solution is an aqueous solution used as Mg2+ ion source in many molecular biology and biochemistry applications. Mg2+ is an essential cofactor in a large list of enzymatic reactions. In PCR reactions, Mg2+ facilitates the binding of primers reducing electrostatic repulsion between to molecules of DNA. For standard PCR, an optimal range is between 1.5 to 5 mM final concentration.

Betaine Enhancer Solution (5 M) Overview Betaine Enhancer Solution (5 M) is an effective solution to use as PCR enhancer. It reduces the DNA melting temperature allowing the amplification on high GC sequences and templates with secondary structures difficult to amplify. The addition of betaine at a final concentration of 0.8-1.6M improves the amplification of … Leer más

PCR Reagents & Kits Q-PLUS™-Probe Real Time qPCR Master Mix (2x) Overview Q-PLUS™-Probe Real Time qPCR Master Mix,  is an optimized Master Mix to be used in real time quantitative PCR. The detection involves the prevention of fluorescence of a fluorophore by the close proximity with a quencher and the 5’–3′ exonuclease activity of Taq … Leer más

PCR Reagents & Kits Q-PLUS™-Green Real Time qPCR Master Mix (2x) Overview Q-PLUS™-Green Real Time qPCR Master Mix, is an optimized formula dye-based Master Mix to be used in real time quantitative PCR. The Master contains an antibody based hotstart DNA polymerase, dNTPs, MgCl2, PCR enhancers and stabilization compound. Features Ready to use. Enhanced specificity and … Leer más

PCR Reagents & Kits WARM™ HotStart PCR Master Mix (2x) Overview WARM™ HotStart Taq DNA Polymerase Kit is convenient kit that includes the hot-start enzyme WARM™ START Taq DNA polymerase and highly purity dNTPs. WARM™ HotStart Taq DNA polymerase is a thermostable polymerase from Thermus aquaticus produced in Escherichia coli reversibly inactivated by antibody binding. … Leer más

PCR Reagents & Kits WARM™ HotStart Taq DNA Polymerase Kit Overview WARM™ HotStart Taq DNA Polymerase Kit is a convenient kit that includes the hot-start enzyme WARM™ START Taq DNA polymerase and highly purity dNTPs. WARM™ HotStart Taq DNA polymerase is a thermostable polymerase from Thermus aquaticus produced in Escherichia coli reversibly inactivated by antibody … Leer más

PCR Reagents & Kits STOUT™ Green PCR Master Mix (2x) Overview STOUT™ Green PCR Master Mix (2x) is an optimized formulation to enable successful amplification in a process with reduced manipulation. The formula contains MgCl2, pure dNTPs, PCR enhancer and our highly purified thermostable STOUT™ Recombinant Taq DNA Polymerase. The master mix is a dense … Leer más

STOUT™ – Red PCR Master Mix (2x) is an optimized formulation to enable successful amplification in a process with reduced manipulation. The formula contains MgCl2, pure dNTPs, PCR enhancer and our highly purified thermostable STOUT™ Recombinant Taq DNA Polymerase. The master mix is a dense solution for direct loading of PCR products onto agarose gel and it includes a tracking dye to monitor the sample electrophoresis.