PCR Reagents & Kits
WARM™ HotStart Taq DNA Polymerase Kit
Overview
WARM™ HotStart Taq DNA Polymerase Kit is a convenient kit that includes the hot-start enzyme WARM™ START Taq DNA polymerase and highly purity dNTPs. WARM™ HotStart Taq DNA polymerase is a thermostable polymerase from Thermus aquaticus produced in Escherichia coli reversibly inactivated by antibody binding. Once the enzyme is activated it has a strong 5′ to 3′ polymerase activity.
Features
- Enhanced specificity and sensibility, amplifies low copy number targets with reduced non-specific.
- Thermostable half-life at 94 ºC is 40 minutes.
- Suitable for TA cloning purposes, the enzyme adds 3′ adenine at the end of PCR fragment.
- Activation controlled, inactive at low temperature and fully activated at temperature > 70ºC.
- Error Rate 1–20×10−5 errors/bp per cycle.
For Research Use Only. Not for use in diagnostic procedures.
Cat. No. | Format | Conc. | Price |
TBK0035 | 20 U | 5 u/µL | 10€ (Test sample)* |
TBK0036 | 500 U | 5 u/µL | 81€ |
TBK0037 | 2 x 500 U | 5 u/µL | 124€ |
*Test sample available! The test sample price will be refunded on the first official order of the product.
Prices excl. VAT plus shipping costs.
Components | TBK0036 | TBK0037 |
WARM™ HotStart Taq DNA polymerase (5 U/ µL) | 100 μL | 2 x 100 μL |
WARM™ PCR Buffer (10x) | 1.5 mL | 2 x 1.5 mL |
MgCl2 25 mM | 1.5 mL | 2 x 1.5 mL |
High-Q™ dNTPs 10 mM TOTAL | 1 mL | 2 x 1 mL |
- Daily research: standard PCR for targets up 5 kb, recombinant clone or cell line checking, etc.
- Generation of PCR fragments for TA cloning.
- DNA labelling due to capacity to incorporate modified nucleotides.
- Real time PCR to quantify DNA or cDNA targets, gene expression, SNPs.
- Shipped in: Shipped in blue ice.
- Storage: Store at -20 °C.
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