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TOO™ LONG DNA Polymerase Master Mix (2x)

PCR Reagents & Kits

TOO™ LONG DNA Polymerase Master Mix (2x)

Overview

TOO™ LONG DNA Polymerase Master Mix (2x) is an optimized formulation to enable the amplification of targets since 5 kb to 20 kb. It is based in a blended enzyme preparation which combine a polymerase with 3′-5′ exonuclease activity with a polymerase lacking 3′-5′ exonuclease activity. PCR amplification generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products.

Features

  • Efficient long targets amplifications (5-20 kb).
  • Ready to use, avoiding mistakes in PCR reaction preparation.
  • PCR fragments suitable to be cloning in TA-vectors or blunt vectors.
  • Increased yield and fidelity of PCR products.
  • Error Rate 5.6 x10−6 errors/bp per cycle.

For Research Use Only. Not for use in diagnostic procedures.

Cat. No. Volume  No. reactions (50 μL) Price
TBK0048 0.125 mL 5 Reactions 10€ (Test sample)*
TBK0049 2 x 1.25 mL 100 Reactions 76€
TBK0050 4 x 1.25 mL  200 Reactions 131€

*Test sample available! The test sample price will be refunded on the first official order of the product.

Prices excl. VAT plus shipping costs.

How to order?

Components TBK0049 TBK0050
TOO™ Long DNA polymerase Master Mix (2x) 2 x 1.25 mL 4 x 1.25 mL
PCR Grade Water, nuclease free 2 x 2 mL 3 x 2 mL
  • Suitable as a direct replacement for ordinary STOUT™ Recombinant Taq DNA Polymerase in most applications.
  • Generation of PCR fragments for TA or blunt cloning.
  • Amplifications of long target sequences.
  • Sequencing.
  • Shipped in: Shipped in blue ice. 
  • Storage: Store at -20 °C.