PCR Reagents & Kits
TOO™ LONG DNA Polymerase Master Mix (2x)
Overview
TOO™ LONG DNA Polymerase Master Mix (2x) is an optimized formulation to enable the amplification of targets since 5 kb to 20 kb. It is based in a blended enzyme preparation which combine a polymerase with 3′-5′ exonuclease activity with a polymerase lacking 3′-5′ exonuclease activity. PCR amplification generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products.
Features
- Efficient long targets amplifications (5-20 kb).
- Ready to use, avoiding mistakes in PCR reaction preparation.
- PCR fragments suitable to be cloning in TA-vectors or blunt vectors.
- Increased yield and fidelity of PCR products.
- Error Rate 5.6 x10−6 errors/bp per cycle.
For Research Use Only. Not for use in diagnostic procedures.
Cat. No. | Volume | No. reactions (50 μL) | Price |
TBK0048 | 0.125 mL | 5 Reactions | 10€ (Test sample)* |
TBK0049 | 2 x 1.25 mL | 100 Reactions | 76€ |
TBK0050 | 4 x 1.25 mL | 200 Reactions | 131€ |
*Test sample available! The test sample price will be refunded on the first official order of the product.
Prices excl. VAT plus shipping costs.
Components | TBK0049 | TBK0050 |
TOO™ Long DNA polymerase Master Mix (2x) | 2 x 1.25 mL | 4 x 1.25 mL |
PCR Grade Water, nuclease free | 2 x 2 mL | 3 x 2 mL |
- Suitable as a direct replacement for ordinary STOUT™ Recombinant Taq DNA Polymerase in most applications.
- Generation of PCR fragments for TA or blunt cloning.
- Amplifications of long target sequences.
- Sequencing.
- Shipped in: Shipped in blue ice.
- Storage: Store at -20 °C.
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