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Q-PLUS™One Step Green RT-qPCR Master Mix (2x)

PCR Reagents & Kits

Q-PLUS™ One Step Green RT-qPCR Master Mix (2x)

Overview

Q-PLUS™- One-Step Green RT-qPCR Master Mix (2X) allows first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably. The kit includes our Q-PLUS™- Green qPCR Master mix developed for fast PCR, provided as a 2x reaction mixture, which contains all components necessary for real-time PCR, including a green intercalating dye, dNTPs, stabilizers and enhancers. In addition, a separate RT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided.

This kit also includes a separate vial of ROX that can be optionally added to the qPCR reaction Mix. The final concentration of ROX will vary depending on each real-time cycler manufacturer’s specification.

Features

  • High Efficiency in multiplex reactions
  • High Efficiency in GC/AT-rich templates
  • Early Ct values – Rapid extension rate
  • Extreme sensitivity – increased limit of detection
  • Compatible with fast and standard PCR program

For Research Use Only. Not for use in diagnostic procedures.

Cat. No.FormatPrice
TBK00135 Reactions10€ (Test sample)*
TBK0014100 Reactions145€
TBK0015500 Reactions535€

*Test sample available! The test sample price will be refunded on the first official order of the product.

Prices excl. VAT plus shipping costs.

How to order?

ComponentsTBK0014TBK0015
Q-PLUS™- One Step Green RT-qPCR  Master Mix (2x)1 mL5 x 1 mL
ROX reference dye1 vial1 vial
RT Mix100 µL5 x 100 µL
RNase-free  Water1 mL5x 1 mL

 

  • Absolute quantification
  • Gene copy number determination
  • Gene expression analysis
  • Cepheid SmartCycler®
  • CFX96 Touch™ Real-Time PCR Detection System
  • CFX384 Touch™ Real-Time PCR Detection System
  • CFX Connect™ Real-Time PCR Detection System
  • LightCycler® Nano Real-Time PCR System
  • LightCycler® 96 Real-Time PCR System
  • LightCycler® 480 Real-Time PCR System
  • LightCycler® 1536 Real-Time PCR System
  • My IQ™ Instrument
  • MyGo Mini S
  • MyGo Pro
  • QuantStudio™ Instrument
  • Rotor Gene® Q
  • Rotor Gene® 6000.

 For instruments not listed, check with the manufacturer

  • Shipped in: Shipped in blue ice. 
  • Storage: Store at -20 °C.