PCR Reagents & Kits
Q-PLUS™ One Step Green RT-qPCR Master Mix (2x)
Overview
Q-PLUS™- One-Step Green RT-qPCR Master Mix (2X) allows first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably. The kit includes our Q-PLUS™- Green qPCR Master mix developed for fast PCR, provided as a 2x reaction mixture, which contains all components necessary for real-time PCR, including a green intercalating dye, dNTPs, stabilizers and enhancers. In addition, a separate RT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided.
This kit also includes a separate vial of ROX that can be optionally added to the qPCR reaction Mix. The final concentration of ROX will vary depending on each real-time cycler manufacturer’s specification.
Features
- High Efficiency in multiplex reactions
- High Efficiency in GC/AT-rich templates
- Early Ct values – Rapid extension rate
- Extreme sensitivity – increased limit of detection
- Compatible with fast and standard PCR program
For Research Use Only. Not for use in diagnostic procedures.
Cat. No. | Format | Price |
TBK0013 | 5 Reactions | 10€ (Test sample)* |
TBK0014 | 100 Reactions | 145€ |
TBK0015 | 500 Reactions | 535€ |
*Test sample available! The test sample price will be refunded on the first official order of the product.
Prices excl. VAT plus shipping costs.
Components | TBK0014 | TBK0015 |
Q-PLUS™- One Step Green RT-qPCR Master Mix (2x) | 1 mL | 5 x 1 mL |
ROX reference dye | 1 vial | 1 vial |
RT Mix | 100 µL | 5 x 100 µL |
RNase-free Water | 1 mL | 5x 1 mL |
- Absolute quantification
- Gene copy number determination
- Gene expression analysis
- Cepheid SmartCycler®
- CFX96 Touch™ Real-Time PCR Detection System
- CFX384 Touch™ Real-Time PCR Detection System
- CFX Connect™ Real-Time PCR Detection System
- LightCycler® Nano Real-Time PCR System
- LightCycler® 96 Real-Time PCR System
- LightCycler® 480 Real-Time PCR System
- LightCycler® 1536 Real-Time PCR System
- My IQ™ Instrument
- MyGo Mini S
- MyGo Pro
- QuantStudio™ Instrument
- Rotor Gene® Q
- Rotor Gene® 6000.
For instruments not listed, check with the manufacturer
- Shipped in: Shipped in blue ice.
- Storage: Store at -20 °C.
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