PCR Detection Kits
Q-PLUS™-qPCR Mycoplasma Detection Kit
Overview
Q-PLUS™-qPCR Mycoplasma Detection Kit is an optimized 2x Master Mix formulation designed to be used in real-time PCR to detect the presence of mycoplasma contamination in biological materials, such as cell cultures. The Master Mix includes an antibody-based hot-start DNA polymerase, primers, probes, and deoxynucleotide triphosphates dATP, dCTP, dGTP and dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment. The kit utilizes a FAM Probe for the detection of mollicutes and a HEX Probe for the detection of the PCR Positive Control.
With Q-PLUS™-qPCR Mycoplasma Detection Kit, the highly conserved 16s rDNA of more than 130 mollicute species is targeted, covering Mycoplasma, Acholeplasma and even Ureaplasma, whereas genomic eukaryotic DNA is not amplified. To exclude false-negatives due to PCR inhibition, an additional PCR positive control is included in the master mix.
Features
- MORE SENSITIVE than staining methods (detection of less than 10 genomes /reaction).
- SPECIFIC (conserved 16s rDNA target for mollicutes).
- RELIABLE (internal PCR positive control)
- LESS FALSE-POSITIVE RESULTS than enzyme-based methods
- PREMIXED FOR EASE-OF-USE: All PCR reaction components are included; just add DNA samples.
- UNIVERSAL ROX CONCENTRATION for maximum instrument compatibility.
- dUTP for optional UNG treatment.
- FAST results can be obtained in less than 2 hours.
For Research Use Only (RUO).
How to order?| Components | TBK0014 |
| 2X qPCR Mastermix | 1 mL |
| Positive Control | 50 µL |
| RNase-free Water | 1 mL |
Parque Científico Tecnológico Rabanales 21, Edificio Aldebarán, Calle Astrónoma Cecilia Payne, 14014 Córdoba. Spain
info@tiarisbiosciences.com
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