Exo/SAP Kit

High-Q^TM Exo/SAP kit is an enzymatic PCR Clean-Up kit, comprising Exonuclease I (Exo I) and recombinant Shrimp Alkaline Phosphatase (rSAP) in an optimal molar ratio. Unused primers are hydrolyzed by Exo I, whilst rSAP dephosphorylates excess dNTPs.

High-Q^TM Exo/SAP kit is designed to degrade dNTPs and primers in a single reaction tube in only 5 minutes. Both enzymes are subsequently removed by heat-inactivation and DNA is ready for sequencing in 15 minutes. No further treatment is required and recovery is 100%, even for very short PCR products.

Features

• Fast 15 minutes protocol
• Add directly to PCR product
• 100 % Sample Recovery
• Scalable for different reaction sizes
• No interference on downstream applications
• No need for spin columns or magnetic beads

For Research Use Only. Not for use in diagnostic procedures.