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Q-PLUS™- Escherichia coli STEC Detection Kit
Overview
Escherichia coli is a normal gram-negative bacterium in our intestines, but some strains of Escherichia coli produce toxins causing food poisoning. These strains are referred to as STEC/ VTEC (shiga toxin or verotoxin-producing E. coli) or EHEC (enterohaemorrhagic E. coli). The toxins they produce can cause bloody diarrhea and Haemolytic Uremic Syndrome (HUS), a severe complication with the potential for fatality.
Q-PLUS™- Escherichia coli STEC Detection Kit is a Real-Time PCR based on Taqman® probes signaling after the amplification of stx1 and stx2 genes associated with cytotoxicity and eae gene associated with the intestinal epithelium adhesion capability (FAM probe). The kit also includes an Internal Positive Control (ROX probe) for monitoring PCR inhibition and false negative results. Negative Control is used to check the absence of contamination.
Q-PLUS™- Escherichia coli STEC Detection Kit is an excellent tool for a rapid and highly sensitive of STEC E. coli detection in food and feed samples. It allows molecular detection of pathogenic E. coli in samples previously enriched, reducing the time of well-established microbiological diagnostic process.
Features
- Fast and reliable detection of E.coli STEC.
- Highest sensitivity: 1-10 cells per 25 g food sample (5×102 fg genomic DNA) can be detected with 100% specificity.
- 100% inclusivity and 100% exclusivity in tested strains.
- Compatible with all real time thermocyclers.
- Validated according to ISO 22118: 2011.
For Research Use Only (RUO).
Cat. No. | Format | Price |
TBK1047 | 150 reactions (50 rxn for each gene) | – € |
Prices excl. VAT plus shipping costs.
Components | TBK0014 |
Q-PLUS™ Escherichia coli Master Mix | 3x 840 µL |
stx1 Primers & Probe | 105 µL |
stx2 Primers & Probe | 105 µL |
eae Primers & Probe | 105 µL |
Positive Control | 70 µL |
Negative Control | 70 µL |
Escherichia coli STEC detection in food and animal feed after a pre-enrichment step according to ISO 16654: 2001.
- Shipped in: Shipped in blue ice.
- Storage: Store all components at –20°C and avoid repeated freeze and thaw cycles. Protect from light. Prepare aliquots is necessary.
Parque Científico Tecnológico Rabanales 21, Edificio Aldebarán, Calle Astrónoma Cecilia Payne, 14014 Córdoba. Spain
info@tiarisbiosciences.com
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