DL-Dithiothreitol (DTT)
(Molecular Biology Grade)
Overview
DL-Dithiothreitol (DTT) is a well-known reducing agent used in biochemistry and molecular biology approaches. DTT penetrates the cellular membrane to safeguard protein sulfhydryl groups in reduced state, consequently restoring enzyme activity that may have been compromised due to oxidation of these functional groups.
Dithiothreitol can act as a less toxic substitute for 2-mercaptoethanol in most applications; typically, a seven-fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (700 mM).
The optimal pH range for DTT is 7.1-8.0. It is less potent reducing agent with decreasing pH levels (< pH 6.5).
Features
- CAS: 3483-12-3
- Formula: C4H10O2S2
- MW: 154.25 g/mol
Research Use Only. Not for use in diagnostic procedures.
How to order?- Purity ≥98% by HPLC / > 99% by titration.
- Redox potential of -0.33 V at pH 7.
- Highly soluble in water (50 mg/ mL).
- Density, 1.04 g/ mL at 20 °C.
- Melting point: 41-44ºC.
- Boling point: 125 ºC.
- As a reducing agent, it reduces the disulfide bonds of proteins and peptides preventing intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins.
- Denature proteins prior to SDS-PAGE.
- Prevent dimerization or oligomerization in crystallization procedures (5 mM DTT is recommended).
- Oxidizing agent.
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