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Cellular and Biochemical Assays
Annexin V-FITC Apoptosis Detection Kit
Overview
Annexin V-FITC Apoptosis Detection Kit is an effective kit designed to dual detection of apoptotic stage: collapse of asymmetrical distribution of phosphatidylserine (PS) and destabilization of membrane integrity. Annexin V binds with high affinity to PS (Kd ~ 5×10-2) in Ca2+ presence and this binding is detected by its fluorochrome conjugated. Propidium iodide only can enter into late apoptotic or necrotic cells, to bind to DNA.
Features
- Earlier Apoptosis Detection than DNA-based assays.
- Dual detection of early- and late-stage cell apoptosis.
- Versatile, suitable for adhesion and suspension cells.
- Flow cytometry analysis:
Signal Detector | Excitation | Emission | |
Annexin-FITC | FL1 | 488 nm | 518- 530 nm |
Propidium Iodide | FL3 | 535 nm | 617 nm |
For Research Use Only. Not for use in diagnostic procedures.
Cat. No. | Format | Price |
TBK0508 | 20 Assays | 69€ |
TBK0509 | 100 Assays | 220€ |
Prices excl. VAT plus shipping costs.
Components | TBK0508 | TBK0509 |
Annexin V-FITC | 0.1 mL | 0.5 mL |
Annexin Binding Buffer 10x | 10 mL | 50 mL |
Propidium Iodide | 0.3 mL | 1 mL |
- Determination of early-stage cell apoptosis.
- Determination of late-stage cell apoptosis.
- Differentiation of apoptosis and necrosis cells.
- Shipped in: Shipped in blue ice.
- Storage: Store the kit at 4°C. Stable for 12 months.
Parque Científico Tecnológico Rabanales 21, Edificio Aldebarán, Calle Astrónoma Cecilia Payne, 14014 Córdoba. Spain
info@tiarisbiosciences.com
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